Composite

Part:BBa_K416007:Design

Designed by: Russell Durrett   Group: iGEM10_NYU   (2010-10-15)


LexO:pCYC:RBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 453
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 453
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
    Illegal XhoI site found at 41
    Illegal XhoI site found at 203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 453
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 453
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our project, this part was used for the Lex operator immediately upstream of the CYC minimal promoter. We fused the RBS onto the part and submitted it to the Registry so future researchers would not have to fuse the RBS for future constructs.


Source

This part comes from parts sourced from the 2010 initial distribution

References